|
Micro Planar Liquid Chromatography has been developed in 2007 at Institute for Chromatography. It was published in special parts at certain international symposia in 2008 - the latest paper as ppt/pdf file is available through this link:
www.institut-f-chromatographie.de/html/mu-plc-report_2008.html/ NOTE: you should have ADOBE Reader 9.0 or higher on your computer to faster load and see the paper as ppt/pdf file.
The theoretical basics for µ-PLC and the practical reasons why to develop a new micro technique for the still and unchanged important planar chromatography will be available in English language later in 2009.
We are still working on the detailed concept of the new micro technique µ-PLC, its fundamental difference to TLC, HPTLC and OPLC, its qualitative and quantitative application and its up-to-now not believed quantitative precision and accuracy (often a factor ten better than with top instrumental HPTLC of today). µ-PLC is the only planar chromatography technique with a sampling volume range from nl to one full ml. It is up to now the only technique which allows for 100% save critical sample comparison. This is possible as the samples overlap locally each other but by part only in bow areas within a limited plate range, where physics, chemistry and time is absolutely identical for the chromatography of to be compared neighboring samples. This situation never exists with any other technique in chromatography. Thus if in µ-PLC samples differ than they differ by 100 % guarantee. In all other chromatography techniques a difference may be caused by time or any of the very many physical and chemical differences of and inside columns, capillaries or plates. Thus there is always a certain range of systematic errors possible causing false differences ending up this way with open questions in analytical results. Of course: absolute correct taking treating and giving samples is a condition sine qua non - a real analyst is able to do it.
|