Host depletion method for pathogen identification in Sepsis Patients
Sepsis is a severe condition caused by the body's overwhelming response to infection. Identifying the specific pathogens responsible for the infection is crucial for effective treatment and management. The host depletion method, along with the micronbrane filters Devin™️ platform, offers a comprehensive solution for pathogen identification.
Here's a general overview of the process:
Sample collection: A blood sample is collected from the sepsis patient. The blood contains both the host DNA (human) and potential microbial DNA (from pathogens).
Host depletion: The Fractionation Syringe Filter is used to remove human DNA from the sample. This step helps to enrich microbial DNA, making it easier to identify and analyze the pathogens present.
Microbial DNA enrichment: The Microbial DNA Enrichment Kit is employed to further concentrate and enrich microbial DNA from the filtered sample. This step helps improve the sensitivity of pathogen detection.
Sequencing and analysis: The enriched microbial DNA is then subjected to DNA sequencing techniques, such as metagenomic next-generation sequencing (mNGS), to generate the genomic data. The obtained data is then analyzed using bioinformatics tools and databases to identify the pathogens present in the sample.
The Devin™️ platform, along with the Fractionation Syringe Filter and Microbial DNA Enrichment Kit, offers an integrated workflow for efficient and reliable pathogen identification in sepsis patients. It combines the selective removal of host DNA, enrichment of microbial DNA, and advanced sequencing technologies to provide comprehensive insights into the infectious agents involved.